About This Book
Enzyme immobilization refers to the process of fixing enzymes onto a solid support or matrix, thereby
restricting their movement while maintaining their catalytic activity. This technique is crucial in industrial
applications as it enhances the stability, reusability, and efficiency of enzymes. Immobilized enzymes are
widely used in food processing, biofuels production, wastewater treatment, and pharmaceutical
industries, offering significant advantages over free enzymes, including easier separation from the
reaction mixture and the possibility of continuous use in bioreactors. Various methods of enzyme
immobilization include adsorption, covalent bonding, entrapment, and encapsulation. Adsorption
involves physically attaching enzymes to a surface, while covalent bonding creates a strong chemical
bond between the enzyme and the support. Entrapment involves trapping enzymes within a gel matrix,
and encapsulation involves enclosing enzymes in a semi-permeable membrane. Each method offers
distinct benefits, such as improved stability, enhanced catalytic performance, or the ability to recycle
enzymes. Immobilized enzymes show increased resistance to harsh environmental conditions, like
temperature and pH fluctuations, which can denature free enzymes. Additionally, they can be reused
multiple times, reducing operational costs. However, the choice of immobilization technique depends
on factors like enzyme type, desired reaction conditions, and cost-effectiveness, making enzyme
immobilization an important area of research for sustainable and efficient industrial processes. Enzyme
Immobilization explores the techniques, applications, and advancements in the immobilization of
enzymes for industrial and biotechnological purposes.
Contents: 1. Industrial and Environmental Applications of Immobilized Enzymes, 2. Enzyme Kinetics
Theory, 3. Fundamentals of Enzyme Catalysis and Immobilization, 4. Immobilized Enzyme Operation,
5. Fundamentals of Enzyme Reactions and Inhibition Mechanisms, 6. Enzymes in Industry, 7. Therapeutic
Innovations using Immobilized Enzyme Technology, 8. Biomedical Applications in Enzyme
Immobilization.
