About This Book
Biochemistry and Biotechnology: A Laboratory Manual is a practical guide designed for students,
researchers, and professionals working in life sciences. It provides hands-on experience in fundamental
and advanced laboratory techniques used in biochemistry and biotechnology. The manual bridges
theoretical knowledge with practical skills, offering clear instructions and step-by-step protocols for
conducting experiments related to enzymes, proteins, nucleic acids, and microbial cultures. This manual
includes experiments such as protein estimation, enzyme activity assays, DNA isolation, gel
electrophoresis, chromatography, spectrophotometry, and microbial culture techniques. Each
experiment is accompanied by background theory, required materials, procedural steps, and expected
results, helping users understand not only how to perform the technique but also why each step is
important. Safety guidelines and troubleshooting tips are also provided to ensure accuracy and
efficiency in the lab. Emphasizing modern biotechnology applications, the manual introduces
techniques like PCR, restriction digestion, plasmid isolation, and genetic transformation, which are
essential in genetic engineering and molecular biology. With a focus on clarity and educational value, this
manual serves as an essential resource for students and educators in biochemistry, biotechnology, and
related fields, fostering both competence and confidence in laboratory environments. This book is a
comprehensive laboratory manual offering practical experiments in biochemistry and biotechnology for
students and researchers.
Contents: 1. Biotechnology Laboratory Techniques, 2. Quality in Analytical Laboratories, 3. Chemistry at
the Macroscopic Level, 4. Analytical Methods in Science, 5. Evolution of Chromatography within the
Modern Laboratory, 6. Restriction Digestion and Plasmid Isolation, 7. Uses of PCR in Biotechnology and
Medicine, 8. Sequence Profiling Tool, 9. Biotechnological Applications of Industrial Enzymes,
10. Principles of Staining.
